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Test ID LBOR0144 BCR/ABL, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay

Reporting Name

BCR/ABL, p190, Quant, Monitor

Useful For

Monitoring response to therapy in patients with known e1/a2 bcr/abl (p190) fusion forms

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Advisory Information

This test should not be used to screen for bcr/abl fusions at the time of diagnosis; BADX / BCR/ABL1, Qualitative, Diagnostic Assay should be ordered for that purpose.

Shipping Instructions

Refrigerate specimen must arrive within 5 days (120 hours of draw), and ambient specimens must arrive within 3 days (72 hours) of draw. Draw and package specimen as close to shipping time as possible.

Necessary Information

The following information is required:

1. Pertinent clinical history including if the patient has a diagnosis of chronic myelogenous leukemia or other bcr/abl-positive neoplasm

2. Date of collection

3. Specimen source (blood or bone marrow)

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Whole blood


Preferred: EDTA (lavender top)

Acceptable: ACD (yellow top)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.


Specimen Type: Bone marrow


Preferred: EDTA (lavender top)

Acceptable: ACD (yellow top)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 5 days
  Ambient  72 hours

Reference Values

The presence or absence of the BCR/ABL mRNA (bcr/abl) fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of bcr/abl (p190) to abl with conversion to a percentage (ie, bcr/abl (p190) as a percentage of total abl).

Day(s) and Time(s) Performed

Monday through Friday a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81207-BCR/ABL1 (t[9;22]) (eg, chronic myelogenous leukemia) translocation analysis; minor breakpoint, qualitative or quantitative

Analytic Time

4 days

Reject Due To


Mild OK; Gross reject






Moderately to severely clotted

Method Name

Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Secondary ID



1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (

Testing Algorithm

See BCR/ABL1 Ordering Guide for Blood and Bone Marrow in Special Instructions.