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Test ID BLOD5015 Thermal Amplitude


Thermal Cold Antibody Test

Specimen Type/Requirements

Red (no-gel serum) Tube, maintain at 37° C prior to centrifugation and separation

Specimen will be rejected if overt hemolysis

Specimen Volume

Preferred Volume 6.0-8.0 mL whole blood separated
Minimum Volume 3.0 mL whole blood separated



Maintain at 37° C prior to centrifugation and separation of serum from cells.  Allow specimen to clot at 37° C.  Separate and send both cells/clot and serum in separate, labeled tubes. 

Room Temperature 3 days  
Refrigerated 3 days Preferred for Transport
Frozen N/A  

Performed Test Frequency



Gel, Test Tube

Performing Lab

Sanford Laboratories Fargo-Transfusion Services/Blood Bank

CPT Code


Useful For

Thermal amplitude is the temperature range over which an antibody can react.  Cold reactive autoantibodies occasionally exhibit thermal amplitude 20-24° Celsius or higher) and may interfere with routine cell and serum tests.  Physicians may request a thermal amplitude test before performing certain surgical procedures when these antibodies are identified.