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HelpTest ID BLOD1610 Testosterone, Total and Bioavailable, Serum
Test Down Notes
Effective August 22, 2024: This test is temporarily unavailable due to analytic issues. The downtime is expected to be >30 days. Order FFTST as an alternative. See test notification here.
Reporting Name
Testosterone, Total and Bioavail, SUseful For
Recommended second-level test for suspected increases or decreases in physiologically active testosterone:
-Assessment of androgen status in cases with suspected or known sex hormone-binding globulin binding abnormalities
-Assessment of functional circulating testosterone in early pubertal boys and older men
-Assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism but normal total testosterone levels
-Monitoring of testosterone therapy or antiandrogen therapy in older men and in females
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BATS | Testosterone, Bioavailable, S | No | Yes |
| TTST | Testosterone, Total, S | Yes | Yes |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedOrdering Guidance
This is the preferred second-level test for suspected increases or decreases in physiologically active testosterone.
Necessary Information
Patient's age and sex are required.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 14 days | |
| Frozen | 60 days |
Reference Values
TESTOSTERONE, TOTAL:
Males
0-5 months: 75-400 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-130 ng/dL
12-13 years: <7-800 ng/dL
14 years: <7-1,200 ng/dL
15-16 years: 100-1,200 ng/dL
17-18 years: 300-1,200 ng/dL
≥19 years: 240-950 ng/dL
Tanner Stages*
I (prepubertal): <7-20
II: 8-66
III: 26-800
IV: 85-1,200
V (young adult): 300-950
Females
0-5 months: 20-80 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-44 ng/dL
12-16 years: <7-75 ng/dL
17-18 years: 20-75 ng/dL
≥19 years: 8-60 ng/dL
Tanner Stages*
I (prepubertal): <7-20
II: <7-47
III: 17-75
IV: 20-75
V (young adult): 12-60
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±2) years and for girls at a median age of 10.5 (±2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (young adult) should be reached by age 18.
TESTOSTERONE, BIOAVAILABLE:
Males
≤19 years: Not established
20-29 years: 83-257 ng/dL
30-39 years: 72-235 ng/dL
40-49 years: 61-213 ng/dL
50-59 years: 50-190 ng/dL
60-69 years: 40-168 ng/dL
≥70 years: Not established
Females (non-oophorectomized)
≤19 years: not established
20-50 years (on oral estrogen): 0.80-4.0 ng/dL
20-50 years (not on oral estrogen): 0.80-10 ng/dL
>50 years: Not established
Day(s) Performed
Monday through Friday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84403
84410
Report Available
2 to 5 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
TTST: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
BATS: Differential Precipitation/Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)