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Test ID NBLD0405 Legionella species, Molecular Detection, PCR, Varies

Useful For

Sensitive and rapid diagnosis of pneumonia caused by Legionella species

 

The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment.

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Legionella DNA is unlikely.

 

Specimen Type: Lower respiratory

Sources: Bronchoalveolar lavage, bronchial aspirate/brushing/lavage/washing, tracheal/endotracheal secretions/aspirate, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Fresh tissue or biopsy

Sources: Lung, pleura, heart valve, pericardium

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3) - approximately the size of a pencil eraser

Collection Instructions: Aseptically collect a 1 to 2 cm(3) piece of tissue whenever possible

 

Specimen type: Fluid

Sources: Pericardial, pleural, chest, chest tube drainage, thoracentesis, empyema

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Minimum Volume

See Specimen Required

Reject Due To

Tissue in formalin, formaldehyde, or acetone
Formalin-fixed paraffin-embedded (FFPE) block		 Reject

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Day(s) Performed

Monday through Sunday

Report Available

3 days

Method Name

Rapid Polymerase Chain Reaction (PCR)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87801

Reporting Name

Legionella PCR

Reference Values

Not applicable

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID

89564
Sanford Laboratories - Fargo Additional Information:

SANFORD INTERFACE BUILD INFORMATION

Result Code Result Code Description
13602 Specimen Source
13603 Result