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Test ID BLOD1598 Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood

Important Note

Time Sensitive Test:

It is recommended that samples arrive at Mayo Clinic Labs within 24 hours of collection.

Collect specimens in the morning so they are received at Sanford Referrals by Noon, Monday through Friday.

To arrange collection outside this time frame, please contact Sanford Laboratories Client Support.

Useful For

Serial monitoring of CD4 T-cell count in patients who are HIV-positive

 

Follow-up and diagnostic evaluation of primary immunodeficiencies, including severe combined immunodeficiency

 

Immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized

 

Assessment of immune reconstitution post-hematopoietic cell transplantation

 

Early screening of gross quantitative anomalies in lymphocyte subsets in infection or malignancies

 

Absolute quantitation of circulating B cells for diagnosis of patients with chronic lymphocytic leukemia as indicated in the 2008 International Workshop on Chronic Lymphocytic Leukemia guidelines

Specimen Type

Whole Blood EDTA


Ordering Guidance


This assay should not be used for diagnosing lymphocytic malignancies or evaluation of lymphocytosis of unknown etiology. For such cases, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies, which includes a hematopathology review.

 

This assay can be used for absolute quantitation of B cells in patients with chronic lymphocytic leukemia.

 

While this assay can be used to follow patients on B-cell-depleting therapy, like rituximab (eg, Rituxan, Riabni) or ofatumumab (eg, Kesimpta), it may be more reasonable and financially viable to use CD20B / CD20 on B Cells, Blood (includes CD45, CD19 and CD20 markers).



Shipping Instructions


It is recommended that specimens arrive within 24 hours of collection. Collect and package specimen as close to shipping time as possible.



Necessary Information


Date and time of collection are required.



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 52 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Method Name

Flow Cytometry

Reporting Name

QN Lymphocyte Subsets: T, B, and NK

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Secondary ID

9336

CPT Code Information

86355

86357

86359

86360

Reference Values

The appropriate age-related reference values will be provided on the report.

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
Sanford Laboratories - Fargo Additional Information:

 

Sanford Laboratories - Enterprise Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

 Result Code     Result Code Description   
 16415  CD45 LYMPH COUNT, FLOW   
 16416  % CD3 (T CELLS)
 16421  CD3 (T CELLS)
 16419  % CD4 (HELPER CELLS)
 16424  CD4 (HELPER CELLS)
 16420  % CD8 (SUPP'R CELLS)
 16425  CD8 (SUPP'R CELLS)
 16417  % CD19 (B CELLS)
 16422  CD19 (B CELLS)
 16418  % CD16+CD56 (NK CELLS)   
 16423  CD16+CD56 (NK CELLS)
 29396  4/8 RATIO
 16427  COMMENT
 29376  % SAMPLE VIABILITY
 29377  % LYMPHOCYTE VIABILITY