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Test ID BLOD1391 Dihydrorhodamine (DHR) Flow Cytometric Phorbol Myristate Acetate (PMA) Test, Blood

Useful For

Diagnosis of chronic granulomatous disease (CGD), X-linked and autosomal recessive forms, complete myeloperoxidase (MPO) deficiency; monitoring chimerism and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase function posthematopoietic cell transplantation

 

Assessing residual NADPH oxidase activity pretransplant

 

Identification of carrier females for X-linked CGD; assessment of changes in lyonization with age in carrier females

Specimen Type

WB Sodium Heparin


Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668) following the instructions in the mailer.

 

It is recommended that specimens arrive within 24 hours of draw.

 

Samples arriving on the weekend may be canceled.



Necessary Information


Ordering physician name and phone number are required.



Specimen Required


Both a whole blood sodium heparin specimen and a whole blood sodium heparin control specimen from an unrelated, healthy donor are required.

 

Supplies: Ambient Mailer-Critical Specimens Only (T668)

 

Patient:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

 

Normal Control:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated person within an hour of the patient.

2. Label clearly on outermost label normal control.

3. Send specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

Lithium heparin
Green top microtube

Specimen Stability Information

Specimen Type Temperature Time
WB Sodium Heparin Ambient 48 hours

Day(s) and Time(s) Performed

Monday through Friday

Specimen must be received by 4 p.m. on Friday.

Analytic Time

3 days

Method Name

Flow Cytometry

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86352

Reporting Name

DHR Flow PMA, B

Reference Values

Result Name

Unit

Cutoff for defining normal

% PMA ox-DHR+

%

≥95%

MFI PMA ox-DHR+

MFI

≥60

Control % PMA ox-DHR+

%

≥95%

Control MFI PMA ox-DHR+

MFI

≥60

 

The appropriate age-related reference values for Absolute Neutrophil Count will be provided on the report.

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Sanford Laboratories - Fargo Additional Information:

SANFORD INTERFACE BUILD INFORMATION

Result Code Result Code Description
18167 Absolute Neutrophil Count
18168 % PMA OX-DHR+
18169 MFI PMA OX-DHR+
18170 Control Absolute Neutrophil Count
18171 Control % PMA OX-DHR+
18172 Control MFI PMA OX-DHR+
18173 Interpretation