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Test ID BLOD1391 Dihydrorhodamine Flow Cytometric Phorbol Myristate Acetate Test, Blood

Important Note

Time Sensitive Test: Sample needs to be sent to the performing lab the same day as collected.

Send to be received at Sanford Monday through Thursday noon.

 

Contact Sanford Lab Outreach to make arrangements for collection of the sample.

Useful For

Evaluating chronic granulomatous disease (CGD), X-linked and autosomal recessive forms, complete myeloperoxidase deficiency

 

Monitoring chimerism and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase function post-hematopoietic cell transplantation

 

Assessing residual NADPH oxidase activity pretransplant

 

Identifying of female carriers for X-linked CGD

 

Assessing changes in lyonization with age in female carriers

Specimen Type

WB Sodium Heparin


Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box.

 

It is recommended that specimens arrive within 24 hours of collection.

 

Samples arriving on the weekend and observed holidays may be canceled.



Necessary Information


Ordering physician name and phone number are required.



Specimen Required


Both a whole blood sodium heparin specimen and a whole blood sodium heparin control specimen from an unrelated, healthy donor are required.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

 

Patient:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

 

Normal Control:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a control specimen from a normal (healthy), unrelated person within an hour of the patient's specimen collection.

2. Label clearly on outermost label normal control.

3. Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Specimen Stability Information

Specimen Type Temperature Time Special Container
WB Sodium Heparin Ambient 48 hours GREEN TOP/HEP

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Method Name

Flow Cytometry

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86352

Reporting Name

DHR Flow PMA, B

Reference Values

Result Name

Unit

Cutoff for defining normal

% PMA ox-DHR+

%

≥95%

MFI PMA ox-DHR+

MFI

≥60

Control % PMA ox-DHR+

%

≥95%

Control MFI PMA ox-DHR+

MFI

≥60

 

MFI = mean fluorescence intensity

PMA = phorbol myristate acetate

DHR = dihydrorhodamine

 

The appropriate age-related reference values for Absolute Neutrophil Count will be provided on the report.

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Secondary ID

62765
Sanford Laboratories - Fargo Additional Information:

SANFORD INTERFACE BUILD INFORMATION

Result Code Result Code Description
18167 Absolute Neutrophil Count
18168 % PMA OX-DHR+
18169 MFI PMA OX-DHR+
18170 Control Absolute Neutrophil Count
18171 Control % PMA OX-DHR+
18172 Control MFI PMA OX-DHR+
18173 Interpretation