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Test ID BLOD1366 JAK2 V617F Mutation Detection, Blood

Useful For

Aiding in the distinction between a reactive blood cytosis and a chronic myeloproliferative disorder in blood specimens

Specimen Type

Whole Blood EDTA

Shipping Instructions

Specimen must arrive within 7 days (168 hours) of draw.

Specimen Required


Preferred: EDTA (lavender top)

Acceptable: ACD (yellow top)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Specimen Minimum Volume

1 mL

Reject Due To


Mild OK; Gross reject






Moderately to severely clotted

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient (preferred) 7 days
  Refrigerated  7 days

Day(s) and Time(s) Performed

Monday through Friday; 12 p.m.

Analytic Time

2 days

Method Name

Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction (PCR)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant

Reporting Name

JAK2 V617F Mutation Detection, B

Reference Values

An interpretive report will be provided.

Testing Algorithm

The following algorithms are available in Special Instructions:

-Erythrocytosis Evaluation Testing Algorithm

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

Sanford Laboratories - Fargo Additional Information:


Result Code Result Code Description
13300 JAK2 V617F Mutation Detection
20796 JAK2 Result, B