Sign in →

Test ID BLOD0146 Adrenocorticotropic Hormone, Plasma

Useful For

Determining the cause of hypercortisolism and hypocortisolism

Specimen Type

Plasma EDTA

Necessary Information

Separate specimens should be submitted when multiple tests are ordered.

Specimen Required

Patient Preparation: For the 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Ice-cooled, lavender top (EDTA)

Submission Container/Tube: Plastic, 5 mL, aliquot tube

Specimen Volume: 1 mL

Collection Instructions:

1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.

2. Collect with a pre-chilled lavender top (EDTA) tube and transport to the laboratory on ice.

3. Centrifuge at refrigerated temperature within 2 hours and immediately separate plasma from cells.

4. Immediately freeze plasma.

Specimen Minimum Volume

0.75 mL

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 28 days
  Refrigerated  3 hours
  Ambient  2 hours

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Method Name

Electrochemiluminescence Immunoassay

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


Reporting Name

Adrenocorticotropic Hormone, P

Reference Values

7.2-63 pg/mL (a.m. draws)

No established reference values for p.m. draws

Pediatric reference values are the same as adults, as confirmed by peer reviewed literature.


Petersen KE: ACTH in normal children and children with pituitary and adrenal diseases. I. Measurement in plasma by radioimmunoassay-basal values. Acta Paediatr Scand 1981;70:341-345


For SI unit Reference Values, see

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Oncology Test Request (T729)

Secondary ID

Sanford Laboratories - Fargo Additional Information:


Result Code Result Code Description
186 Adrenocorticotropic Hormone