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Test ID NBLD0457 Epstein-Barr Virus (EBV), Molecular Detection, PCR

Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens for laboratory diagnosis of disease due to this virus

Specimen Type


Necessary Information

Specimen source is required.

Specimen Required

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis, amniotic, or ocular

Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.


Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate


Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile container

Specimen Volume: 1.5 mL



Culturette (BBL Culture Swab) (T092)

M4-RT (T605)

Specimen Type: Swab

Sources: Eye swabs and upper respiratory swabs (nasal, throat)

Container/Tube: Multimicrobe media (M4-RT)

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)


Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) only

Specimen Volume: 0.5 mL

Additional Information: Clotted specimens will be rejected.


Supplies: M4-RT (T605)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Preferred: Multimicrobe medium (M4-RT)

Acceptable: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.

Specimen Minimum Volume

Body Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy

Reject Due To








Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and paraffin-embedded tissues

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.

Analytic Time

Monday through Friday: 2 days; Saturday, Sunday: 3 days

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


Reporting Name

Epstein-Barr Virus PCR

Reference Values


Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID

Sanford Laboratories - Fargo Additional Information:


Result Code Result Code Description
13463 Specimen Source
13464 Epstein-Barr Virus PCR