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Test ID LBOR0164 Nucleophosmin (NPM1) Mutation Analysis

Useful For

As a prognostic indicator in patients with newly diagnosed acute myelogenous leukemia with normal karyotype and no FLT3 mutation

Specimen Type

Varies


Shipping Instructions


Specimen must arrive within 7 days (168 hours) of draw.



Necessary Information


The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date of collection

4. Specimen source



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube: EDTA (lavender top) or ACD-B (yellow top)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube: EDTA (lavender top) or ACD-B (yellow top)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

 

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube with indication of volume and concentration of the DNA

Specimen Volume: Entire specimen

Collection Instructions: Label specimen as extracted DNA from blood or bone marrow.


Specimen Minimum Volume

Blood, Bone Marrow: 0.5
Extracted DNA from Blood or Bone Marrow: 10 microliter at 20 ng/microliter

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

Bone marrow biopsies, paraffin-embedded bone marrow clots, slides, paraffin shavings, or moderately to severely clotted

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred) 7 days
  Refrigerated  7 days

Day(s) and Time(s) Performed

Monday through Friday; 3 p.m.

Analytic Time

4 days

Method Name

DNA Polymerase Chain Reaction (PCR) with Fragment Analysis by Capillary Gel Electrophoresis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81310-NPM1 (nucleophosmin) (eg, acute myeloid leukemia) gene analysis; exon 12 variants

Reporting Name

Nucleophosmin Mutation Analysis

Reference Values

An interpretive report will be provided.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Secondary ID

89292

Forms

1. Hematopathology Patient Information Sheet (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Sanford Laboratories - Fargo Additional Information:

SANFORD INTERFACE BUILD INFORMATION

Result Code Result Code Description
21734 Specimen
21735 Final Diagnosis