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Test ID BLOD0923 Direct Antiglobulin Test

AKA

DAT

DATP

DATG

Direct Coombs

Poly

Specimen Type/Requirements

EDTA Specimen - pink or lavender tubes

Specimen will be rejected if overt hemolysis

Specimens need to be received and tested within 48 hours of specimen collection

Performed Test Frequency

Daily, All Shifts

Methodology

Test Tube, Gel

Performing Lab

Sanford Fargo

CPT

86880

Storage Onsite

Refrigerate 1-10°C (tubes are held 3-14 days post transfusion)

Transport Temperature

Send tube(s) room temperature of refrigerated (1-10°C)

Notes:

Reference Value: Negative

If positive, additional testing with anti-IgG and anti C3b3d, eluate, and antibody identification may be done with physician order.

Minimum Volume

Adult:  3.0 ml pink or full lavender tube

Pediatric:  1.0 ml EDTA whole blood