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Test ID BLOD0923 Direct Antiglobulin Test

AKA

DAT

DATP

DATG

Direct Coombs

Poly

Specimen Type/Requirements

EDTA Specimen - pink or lavender tubes

Specimen will be rejected if overt hemolysis

Specimens need to be received and tested within 48 hours of specimen collection

Minimum Volume

Adult:  3.0 ml pink or full lavender tube

Pediatric:  1.0 ml EDTA whole blood

Transport Temperature

Send tube(s) room temperature of refrigerated (1-10°C)

Performed Test Frequency

Daily, All Shifts

Methodology

Test Tube, Gel

CPT

86880

Storage Onsite

Refrigerate 1-10°C (tubes are held 3-14 days post transfusion)

Performing Lab

Sanford Fargo

Notes:

Reference Value: Negative

If positive, additional testing with anti-IgG and anti C3b3d, eluate, and antibody identification may be done with physician order.