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HelpTest ID BLOD0436 Fatty Acid Profile, Essential, Serum
Useful For
Evaluating the nutritional intake and intestinal absorption of essential fatty acids using serum specimens
Identifying deficiency of essential and other nutritionally beneficial fatty acids
Monitoring treatment of patients with essential fatty acid deficiencies who are receiving linoleic acid (C18:2w6) and alpha-linolenic acid (C18:3w3)
Specimen Type
SerumNecessary Information
1. Patient's age is required.
2. Include information regarding treatment, family history, and tentative diagnosis.
Specimen Required
Patient Preparation:
1. For nutritional assessment, patient should fast overnight (12-14 hours); for patients with a suspected fatty acid oxidation disorder, collect immediately before next feeding as fasting is contraindicated.
2. Patient must not consume any alcohol for 24 hours before the specimen collection.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.15 mL
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 92 days | |
| Refrigerated | 72 hours |
Day(s) Performed
Monday through Friday
Report Available
3 to 5 daysMethod Name
Gas Chromatography Mass Spectrometry (GC-MS) Stable Isotope Dilution Analysis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82725
Reporting Name
Fatty Acid Profile, Essential, SReference Values
Lauric Acid, C12:0
<1 year: 6-190 nmol/mL
1-17 years: 5-80 nmol/mL
≥18 years: 6-90 nmol/mL
Myristic Acid, C14:0
<1 year: 30-320 nmol/mL
1-17 years: 40-290 nmol/mL
≥18 years: 30-450 nmol/mL
Hexadecenoic Acid, C16:1w9
<1 year: 21-69 nmol/mL
1-17 years: 24-82 nmol/mL
≥18 years: 25-105 nmol/mL
Palmitoleic Acid, C16:1w7
<1 year: 20-1,020 nmol/mL
1-17 years: 100-670 nmol/mL
≥18 years: 110-1,130 nmol/mL
Palmitic Acid, C16:0
<1 year: 720-3,120 nmol/mL
1-17 years: 960-3,460 nmol/mL
≥18 years: 1,480-3,730 nmol/mL
Gamma-Linolenic Acid, C18:3w6
<1 year: 6-110 nmol/mL
1-17 years: 9-130 nmol/mL
≥18 years: 16-150 nmol/mL
Alpha-Linolenic Acid, C18:3w3
<1 year: 10-190 nmol/mL
1-17 years: 20-120 nmol/mL
≥18 years: 50-130 nmol/mL
Linoleic Acid, C18:2w6
≤31 days: 350-2,660 nmol/mL
32 days-11 months: 1,000-3,300 nmol/mL
1-17 years: 1,600-3,500 nmol/mL
≥18 years: 2,270-3,850 nmol/mL
Oleic Acid, C18:1w9
<1 year: 250-3,500 nmol/mL
1-17 years: 350-3,500 nmol/mL
≥18 years: 650-3,500 nmol/mL
Vaccenic Acid, C18:1w7
<1 year: 140-720 nmol/mL
1-17 years: 320-900 nmol/mL
≥18 years: 280-740 nmol/mL
Stearic Acid, C18:0
<1 year: 270-1,140 nmol/mL
1-17 years: 280-1,170 nmol/mL
≥18 years: 590-1,170 nmol/mL
EPA, C20:5w3
<1 year: 2-60 nmol/mL
1-17 years: 8-90 nmol/mL
≥18 years: 14-100 nmol/mL
Arachidonic Acid, C20:4w6
<1 year: 110-1,110 nmol/mL
1-17 years: 350-1,030 nmol/mL
≥18 years: 520-1,490 nmol/mL
Mead Acid, C20:3w9
≤31 days: 8-60 nmol/mL
32 days-11 months: 3-24 nmol/mL
1-17 years: 7-30 nmol/mL
≥18 years: 7-30 nmol/mL
Homo-Gamma-Linolenic C20:3w6
<1 year: 30-170 nmol/mL
1-17 years: 60-220 nmol/mL
≥18 years: 50-250 nmol/mL
Arachidic Acid, C20:0
<1 year: 30-120 nmol/mL
1-17 years: 30-90 nmol/mL
≥18 years: 50-90 nmol/mL
DHA, C22:6w3
<1 year: 10-220 nmol/mL
1-17 years: 30-160 nmol/mL
≥18 years: 30-250 nmol/mL
DPA, C22:5w6
<1 year: 3-70 nmol/mL
1-17 years: 10-50 nmol/mL
≥18 years: 10-70 nmol/mL
DPA, C22:5w3
<1 year: 6-110 nmol/mL
1-17 years: 30-270 nmol/mL
≥18 years: 20-210 nmol/mL
DTA, C22:4w6
<1 year: 2-50 nmol/mL
1-17 years: 10-40 nmol/mL
≥18 years: 10-80 nmol/mL
Docosenoic Acid, C22:1
<1 year: 2-20 nmol/mL
1-17 years: 4-13 nmol/mL
≥18 years: 4-13 nmol/mL
Nervonic Acid, C24:1w9
<1 year: 30-150 nmol/mL
1-17 years: 50-130 nmol/mL
≥18 years: 60-100 nmol/mL
Triene/Tetraene Ratio
≤31 days: 0.017-0.083
32 days-17 years: 0.013-0.050
≥18 years: 0.010-0.038
Total Saturated Acid
<1 year: 1.2-4.6 mmol/L
1-17 years: 1.4-4.9 mmol/L
≥18 years: 2.5-5.5 mmol/L
Total Monounsaturated Acid
<1 year: 0.3-4.6 mmol/L
1-17 years: 0.5-4.4 mmol/L
≥18 years: 1.3-5.8 mmol/L
Total Polyunsaturated Acid
<1 year: 1.1-4.9 mmol/L
1-17 years: 1.7-5.3 mmol/L
≥18 years: 3.2-5.8 mmol/L
Total w3
<1 year: 0.0-0.4 mmol/L
1-17 years: 0.1-0.5 mmol/L
≥18 years: 0.2-0.5 mmol/L
Total w6
<1 year: 0.9-4.4 mmol/L
1-17 years: 1.6-4.7 mmol/L
≥18 years: 3.0-5.4 mmol/L
Total Fatty Acids
<1 year: 3.3-14.0 mmol/L
1-17 years: 4.4-14.3 mmol/L
≥18 years: 7.3-16.8 mmol/L
Secondary ID
82426Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.SANFORD INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 13239 | Lauric Acid, C12:0 |
| 13240 | Myristic Acid, C14:0 |
| 13241 | Hexadecenoic Acid, C16:1W9 |
| 13242 | Palmitoleic Acid, C16:1W7 |
| 13243 | Palmitic Acid, C16:0 |
| 13244 | G-Linolenic Acid, C18:3W6 |
| 13245 | A-Linolenic Acid, C18:3W6 |
| 13246 | Linoleic Acid, C18:2W6 |
| 134247 | Oleic Acid, C18:1W9 |
| 13248 | Vaccenic Acid, C18:1W7 |
| 13249 | Stearic Acid, C18:0 |
| 13250 | EPA, C20:5W3 |
| 13251 | Arachidonic Acid, C20:4W6 |
| 13252 | Mead Acid, C20:3W9 |
| 13253 | H-G-Linolenic Acid, C20:3W6 |
| 13254 | Arachidic Acid, C20:0 |
| 13255 | DHA, C22:6W3 |
| 13256 | DPA, C22:5W6 |
| 13257 | DPA, C22:5W3 |
| 13258 | DTA, C22:4W6 |
| 13259 | Docosenoic Acid, C22:1 |
| 13260 | Nervonic Acid, C24:1W9 |
| 13261 | Triene Tetraene Ratio |
| 13262 | Total Saturated Acid |
| 13263 | Total Monosaturated Acid |
| 13264 | Total Polyunsaturated Acid |
| 13265 | Total W3 |
| 13266 | Total W6 |
| 13267 | Total Fatty Acids |
| 13268 | Interpretation |